A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Identifieur interne : 000243 ( Main/Exploration ); précédent : 000242; suivant : 000244A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Auteurs : Nikko P. Torres [Canada] ; Grant W. BrownSource :
- Methods in molecular biology (Clifton, N.J.) ; 2015.
English descriptors
- KwdEn :
- MESH :
- cytology : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Microscopy, Confocal, Microscopy, Fluorescence.
- Cells, Cultured, DNA Replication, Imaging, Three-Dimensional, Stress, Physiological, Time Factors.
Abstract
High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
DOI: 10.1007/978-1-4939-2596-4_1
PubMed: 25916702
Affiliations:
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Le document en format XML
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